Lentiviral Production in Wave Bioreactor (2015): Suspension cell culture is used to produce large quantities of Lentiviral vector. The rocking platform generates a 'wave' in the disposable bag which keeps cells in suspension.
A cake that only some of us got to enjoy!
A pellet of E.coli containing a plasmid expressing a pink fluorescent protein.
E.coli from a large scale industrial production of our clinical trial plasmid pGM169.
Proposed 3D model of the CFTR protein.
DNA fragments being cut from an agarose gel exposed to UV.
A CFTR Western blot, to confirm protein production in cell culture.
Pellets of DNA following precipitation.
Light microscope image of a human airway liquid interface cultures. Dark patches are mucous.
Mouse lung large airway (cell nuclei blue) transduced with an adenoviral vector (green).
Human airway liquid interface cultures transduced with a lentivirus expressing Luciferase.
Sheep lung parenchyma (cell nuclei blue) transduced with an adenoviral vector (green).
A frozen vial of GL67A (left) and a frozen vial of pGM169 plasmid DNA (right)
Schematic diagram of the large human airways.
Purifying mRNA from tissue samples.
Large scale lentivirus production in suspension culture.