Lentiviral Production in Wave Bioreactor (2015): Suspension cell culture is used to produce large quantities of Lentiviral vector. The rocking platform generates a 'wave' in the disposable bag which keeps cells in suspension.
Mouse lung large airway (cell nuclei blue) transduced with an adenoviral vector (green).
Light microscope image of a human airway liquid interface cultures. Dark patches are mucous.
Pellets of DNA following precipitation.
E.coli from a large scale industrial production of our clinical trial plasmid pGM169.
Schematic diagram of the large human airways.
Large scale lentivirus production in suspension culture.
A frozen vial of GL67A (left) and a frozen vial of pGM169 plasmid DNA (right)
A cake that only some of us got to enjoy!
Proposed 3D model of the CFTR protein.
DNA fragments being cut from an agarose gel exposed to UV.
Sheep lung parenchyma (cell nuclei blue) transduced with an adenoviral vector (green).
A CFTR Western blot, to confirm protein production in cell culture.
A pellet of E.coli containing a plasmid expressing a pink fluorescent protein.
Purifying mRNA from tissue samples.
Human airway liquid interface cultures transduced with a lentivirus expressing Luciferase.