Time Lapse of Lentivirus Vector Harvesting
Harvesting cells that have been used to produce a large scale prep of lentivirus.
Dr Ian Pringle, Wadham College Talk.
Dr Ian Pringle, gives a talk to the Wadham College Medical Society.
DNA Columns Being Washed
One the most routine things we do in the lab is make plasmid DNA. Here the DNA, bound to a column is being washed.
Lentiviral Production in Wave Bioreactor
Suspension cell culture is used to produce large quantities of Lentiviral vector. The rocking platform generates a 'wave' in the disposable bag which keeps cells in suspension.
Cutting Fragments out of an Agarose Gel Under UV
When making novel plasmid DNA constructs, we have to cut DNA fragments out of agarose gels. DNA fragments separate by size on the gel and so can be identified easily when exposed to UV.
Presentation of Multi Dose Clinical Trial Results
Prof Eric Alton presents the data from our MD clinical trial to an invited audience from the UK CF community. May 30 2015, Imperial College, London.
Prof Jane Davies: Current issues around LCI (Lung Clearance Index).
Prof Jane Davies addresses the the UK Cystic Fibrosis Conference 2015 in Manchester.
Prof Eric Alton: Moving gene therapy into CF patients.
Prof Eric Alton addresses the UK Cystic Fibrosis Conference 2015 in Manchester.
STV News Report on our Multi Dose Clinical Trial.
Report from STV following the publication of results from our clinical trial.
Channel 5 News Report on our Multi Dose Clinical Trial.
Report from Channel 5 News on our clinical trial results.
Western Blot (SDS-PAGE) Time Lapse
A time lapse video of proteins being separated on an SDS-PAGE gel.
CF Unite: CF Genes and Genetics
Dr Ian Pringle discusses CF genes and how new technology is speeding up research and discovery.
CF Unite: Where are we now and where are we going?
Dr Uta Griesenbach discusses the current gene therapy trial, the work leading up to it, and what may be just around the corner.
An Introduction to our Multi-Dose Clinical Trial
A number of our Edinburgh team discuss the clinical trial.
Just Gene Therapy Lecture, 11 September 2012
Prof Eric Alton summaries the aims of the UK CFGTC and our progress to date.
Suspension Cell Culture
HEK293T cells growing in small scale suspension culture for Lentivirus production.
Animation of LV Pseudotyping
Short animation describing why we have to modify lentiviral vectors to allow them to transduce airway cells.
Medical Futures Innovation Awards 2011
Dr Uta Griesenbach talks about CF gene therapy and our viral gene therapy programme.
Basic description of Lentivirus Pseudotyping.
Dr Uta Griesenbach talks about our Lentiviral Research Programme.
Brief introduction to our Lentivirus work, filmed around the time of our two wins at the 2011 Medical Futures Innovation Awards.
Aerosol Gene Delivery.
An Aerotech II nebuliser generating a PEI/pDNA aerosol.
Neutralisation of a bacterial lysate.
Prof Eric Alton
Prof Eric Alton (Imperial) gave this interview to the CF Trust following a public meeting on our work in late 2009.
Gl67A Mixing Device
Mixing device developed by the Consortium to reproducibly mix GL67A and pGM169 for the clinical trial.
In this video Dr Gill introduces the group and talks about why she became involved in this area of research.
In this video, Dr Gill explains how improvements in conventional treatments for CF have greatly improved the level of care available for people with the disease.
Human Airway Epithelia Viewed by UltraView Microscope.
The UltraView microscope is a powerful confocal microscope than can scan through multiple layers of cells in 3D.
What is Gene Therapy?
CF gene therapy aims to insert a fully functional CFTR gene into the lung airway cells of people with the condition. Two main types of vector are used to deliver this new copy of the gene. A viral vector, engineered from viral particles or non-viral vectors, plasmid DNA molecules condensed by other chemical entities.
Our Biomek NX is used to purify RNA/DNA from samples and also to setup a range of assays in the lab. The precision of the 'robot' is greater than human pipetting and allows us to massively increase the throughput of sample processing.