Lentiviral Production in Wave Bioreactor (2015): Suspension cell culture is used to produce large quantities of Lentiviral vector. The rocking platform generates a 'wave' in the disposable bag which keeps cells in suspension.
E.coli from a large scale industrial production of our clinical trial plasmid pGM169.
Large scale lentivirus production in suspension culture.
Pellets of DNA following precipitation.
A pellet of E.coli containing a plasmid expressing a pink fluorescent protein.
Light microscope image of a human airway liquid interface cultures. Dark patches are mucous.
DNA fragments being cut from an agarose gel exposed to UV.
Proposed 3D model of the CFTR protein.
Schematic diagram of the large human airways.
Mouse lung large airway (cell nuclei blue) transduced with an adenoviral vector (green).
A CFTR Western blot, to confirm protein production in cell culture.
Purifying mRNA from tissue samples.
Human airway liquid interface cultures transduced with a lentivirus expressing Luciferase.
A frozen vial of GL67A (left) and a frozen vial of pGM169 plasmid DNA (right)
A cake that only some of us got to enjoy!
Sheep lung parenchyma (cell nuclei blue) transduced with an adenoviral vector (green).