Cutting Fragments out of an Agarose Gel Under UV (2015): When making novel plasmid DNA constructs, we have to cut DNA fragments out of agarose gels. DNA fragments separate by size on the gel and so can be identified easily when exposed to UV.
A cake that only some of us got to enjoy!
Proposed 3D model of the CFTR protein.
Pellets of DNA following precipitation.
Human airway liquid interface cultures transduced with a lentivirus expressing Luciferase.
Light microscope image of a human airway liquid interface cultures. Dark patches are mucous.
Schematic diagram of the large human airways.
A CFTR Western blot, to confirm protein production in cell culture.
Purifying mRNA from tissue samples.
Mouse lung large airway (cell nuclei blue) transduced with an adenoviral vector (green).
Large scale lentivirus production in suspension culture.
A pellet of E.coli containing a plasmid expressing a pink fluorescent protein.
E.coli from a large scale industrial production of our clinical trial plasmid pGM169.
DNA fragments being cut from an agarose gel exposed to UV.
A frozen vial of GL67A (left) and a frozen vial of pGM169 plasmid DNA (right)
Sheep lung parenchyma (cell nuclei blue) transduced with an adenoviral vector (green).