Cutting Fragments out of an Agarose Gel Under UV (2015): When making novel plasmid DNA constructs, we have to cut DNA fragments out of agarose gels. DNA fragments separate by size on the gel and so can be identified easily when exposed to UV.
A cake that only some of us got to enjoy!
Pellets of DNA following precipitation.
Sheep lung parenchyma (cell nuclei blue) transduced with an adenoviral vector (green).
Purifying mRNA from tissue samples.
Human airway liquid interface cultures transduced with a lentivirus expressing Luciferase.
A CFTR Western blot, to confirm protein production in cell culture.
DNA fragments being cut from an agarose gel exposed to UV.
Mouse lung large airway (cell nuclei blue) transduced with an adenoviral vector (green).
A pellet of E.coli containing a plasmid expressing a pink fluorescent protein.
Large scale lentivirus production in suspension culture.
Light microscope image of a human airway liquid interface cultures. Dark patches are mucous.
Proposed 3D model of the CFTR protein.
Schematic diagram of the large human airways.
E.coli from a large scale industrial production of our clinical trial plasmid pGM169.
A frozen vial of GL67A (left) and a frozen vial of pGM169 plasmid DNA (right)