Papers 5

  1. The significance of plasmid DNA preparations contaminated with bacterial genomic DNA on inflammatory responses following delivery of lipoplexes to the murine lung.
    Bazzani RP et al., Biomaterials. 2011 Dec;32(36):9854-65. doi: 10.1016/j.biomaterials.2011.08.092. Epub 2011 Sep 23.
  2. Transgene sequences free of CG dinucleotides lead to high level, long-term expression in the lung independent of plasmid backbone design.
    Bazzani RP et al., 2016 Jul;93:20-6.
  3. Strategies for long-term expression of transgenes in the respiratory epithelium.
    Gill DR et al., Curr Opin Mol Ther. 2010 Aug;12(4):386-93.
  4. The use of CpG-free plasmids to mediate persistent gene expression following repeated aerosol delivery of pDNA/PEI complexes.
    Davies LA et al., Biomaterials. 2012 Aug;33(22):5618-27. doi: 10.1016/j.biomaterials.2012.04.019. Epub 2012 May 8.
  5. CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.
    Hyde SC et al., Nat Biotechnol. 2008 May;26(5):549-51. doi: 10.1038/nbt1399. Epub 2008 Apr 27.

Abstracts 6

  1. CpG-Dependent Inflammatory Response after Delivery of Lipid/pDNA Complexes to Murine Lungs.
    Bazzani RP et al.,The American Society of Gene Therapy Annual Conference (2007)
  2. The Effect of pDNA Quality on Gene Transfer Outcome In Vivo.
    Bazzani RP et al.,The American Society of Gene Therapy Annual Conference (2009)
  3. Persistent gene expression in the murine lung is dependent on transgene CpG content.
    Oliveira C et al.,3rd Minicircle & DNA Vector Conference. (2014)
  4. Minicircles Are Similar To Plasmids In Providing High Level, Long-Term Expression In The Lung .
    Gill DR et al.,The American Society of Gene and Cell Therapy Annual Conference (2016)
  5. Repeated Exposure to pDNA/PEI Aerosols Results in Minimal Detectable Toxicity in the Mouse Lungs .
    Nunez-Alonso GA et al.,The American Society of Gene Therapy Annual Conference (2009)
  6. Genomic DNA Reduction for Therapeutic DNA Manufacture.
    Cai Y et al.,The American Society of Gene and Cell Therapy Annual Conference (2010)

 

Schematic diagram of the large human airways.

 

A frozen vial of GL67A (left) and a frozen vial of pGM169 plasmid DNA (right)