Lentiviral Production in Wave Bioreactor (2015): Suspension cell culture is used to produce large quantities of Lentiviral vector. The rocking platform generates a 'wave' in the disposable bag which keeps cells in suspension.
Light microscope image of a human airway liquid interface cultures. Dark patches are mucous.
E.coli from a large scale industrial production of our clinical trial plasmid pGM169.
A CFTR Western blot, to confirm protein production in cell culture.
A pellet of E.coli containing a plasmid expressing a pink fluorescent protein.
Human airway liquid interface cultures transduced with a lentivirus expressing Luciferase.
Schematic diagram of the large human airways.
Purifying mRNA from tissue samples.
Large scale lentivirus production in suspension culture.
Sheep lung parenchyma (cell nuclei blue) transduced with an adenoviral vector (green).
Mouse lung large airway (cell nuclei blue) transduced with an adenoviral vector (green).
A frozen vial of GL67A (left) and a frozen vial of pGM169 plasmid DNA (right)
A cake that only some of us got to enjoy!
DNA fragments being cut from an agarose gel exposed to UV.
Pellets of DNA following precipitation.
Proposed 3D model of the CFTR protein.