Lentiviral Production in Wave Bioreactor (2015): Suspension cell culture is used to produce large quantities of Lentiviral vector. The rocking platform generates a 'wave' in the disposable bag which keeps cells in suspension.
Proposed 3D model of the CFTR protein.
DNA fragments being cut from an agarose gel exposed to UV.
A frozen vial of GL67A (left) and a frozen vial of pGM169 plasmid DNA (right)
A CFTR Western blot, to confirm protein production in cell culture.
Pellets of DNA following precipitation.
Sheep lung parenchyma (cell nuclei blue) transduced with an adenoviral vector (green).
Schematic diagram of the large human airways.
Purifying mRNA from tissue samples.
Human airway liquid interface cultures transduced with a lentivirus expressing Luciferase.
Light microscope image of a human airway liquid interface cultures. Dark patches are mucous.
Mouse lung large airway (cell nuclei blue) transduced with an adenoviral vector (green).
Large scale lentivirus production in suspension culture.
A pellet of E.coli containing a plasmid expressing a pink fluorescent protein.
E.coli from a large scale industrial production of our clinical trial plasmid pGM169.
A cake that only some of us got to enjoy!