What is Gene Therapy? (2008): CF gene therapy aims to insert a fully functional CFTR gene into the lung airway cells of people with the condition. Two main types of vector are used to deliver this new copy of the gene. A viral vector, engineered from viral particles or non-viral vectors, plasmid DNA molecules condensed by other chemical entities. More...
Pellets of DNA following precipitation.
Purifying mRNA from tissue samples.
A pellet of E.coli containing a plasmid expressing a pink fluorescent protein.
Large scale lentivirus production in suspension culture.
E.coli from a large scale industrial production of our clinical trial plasmid pGM169.
A frozen vial of GL67A (left) and a frozen vial of pGM169 plasmid DNA (right)
Schematic diagram of the large human airways.
DNA fragments being cut from an agarose gel exposed to UV.
Light microscope image of a human airway liquid interface cultures. Dark patches are mucous.
Proposed 3D model of the CFTR protein.
Sheep lung parenchyma (cell nuclei blue) transduced with an adenoviral vector (green).
A CFTR Western blot, to confirm protein production in cell culture.
Human airway liquid interface cultures transduced with a lentivirus expressing Luciferase.
A cake that only some of us got to enjoy!
Mouse lung large airway (cell nuclei blue) transduced with an adenoviral vector (green).