Lentiviral Production in Wave Bioreactor (2015): Suspension cell culture is used to produce large quantities of Lentiviral vector. The rocking platform generates a 'wave' in the disposable bag which keeps cells in suspension.
A cake that only some of us got to enjoy!
Mouse lung large airway (cell nuclei blue) transduced with an adenoviral vector (green).
Sheep lung parenchyma (cell nuclei blue) transduced with an adenoviral vector (green).
Human airway liquid interface cultures transduced with a lentivirus expressing Luciferase.
Pellets of DNA following precipitation.
A pellet of E.coli containing a plasmid expressing a pink fluorescent protein.
A frozen vial of GL67A (left) and a frozen vial of pGM169 plasmid DNA (right)
E.coli from a large scale industrial production of our clinical trial plasmid pGM169.
A CFTR Western blot, to confirm protein production in cell culture.
Schematic diagram of the large human airways.
Large scale lentivirus production in suspension culture.
Proposed 3D model of the CFTR protein.
Light microscope image of a human airway liquid interface cultures. Dark patches are mucous.
DNA fragments being cut from an agarose gel exposed to UV.
Purifying mRNA from tissue samples.