A novel mixing device for the reproducible generation of nonviral gene therapy formulations.

Davies LA, Nunez-Alonso GA, Hebel HL, Scheule RK, Cheng SH, Hyde SC, Gill DR

BioTechniques

Biotechniques. 2010 Sep;49(3):666-8. doi: 10.2144/000113498.

 Download the Manuscript

Contact us about this publication

Print this page

Nonviral gene therapy utilizing plasmid DNA (pDNA) complexed with cationic lipids (lipoplexes) or cationic polymers (polyplexes) has demonstrated considerable potential for the treatment of a variety of diseases.

However, progress toward clinical application is often delayed by the lack of reliable and scalable mixing of components sufficient to guarantee consistent performance in vivo.

Attempts to improve and standardize mixing have been limited by the sensitivity of pDNA to shear-related degradation. Here we describe a simple pneumatic mixing device that enables the rapid and reproducible production of large volumes of nonviral gene therapy formulations and demonstrate its suitability for use with shear-sensitive pDNA.

 

A novel mixing device

Go Back to Publications     View on Pubmed (20854270)    View on Google Scholar